qPCR Data Analysis R package rtpcr version 2.1.8 Tools for qPCR data analysis using Delta Ct and Delta Delta Ct methods, including t-test, Wilcoxon-test, ANOVA models, and publication-ready visualizations. The package supports multiple target, and multiple reference genes, and uses a calculation framework adopted from Ganger et al. (2017) <doi:10.1186/s12859-017-1949-5> and Taylor et al. (2019) <doi:10.1016/j.tibtech.2018.12.002>, covering both the Livak and Pfaffl methods. Software Depends: R (>= 3.5.0) Imports: multcomp, ggplot2, lme4, lmerTest, purrr, reshape2, tidyr, dplyr, grid, emmeans, lifecycle Suggests: knitr, rmarkdown, multcompView Ghader Mirzaghaderi <mirzaghaderi@gmail.com> Comprehensive R Archive Network (CRAN) Ghader Mirzaghaderi [aut, cre, cph] GPL-3 2026-05-21 application/tgz https://CRAN.R-project.org/package=rtpcr doi:10.32614/CRAN.package.rtpcr