Genotype Calling with Uncertainty from Sequencing Data in Polyploids and Diploids R package polyRAD version 2.0.1 Read depth data from genotyping-by-sequencing (GBS) or restriction site-associated DNA sequencing (RAD-seq) are imported and used to make Bayesian probability estimates of genotypes in polyploids or diploids. The genotype probabilities, posterior mean genotypes, or most probable genotypes can then be exported for downstream analysis. 'polyRAD' is described by Clark et al. (2019) <doi:10.1534/g3.118.200913>, and the Hind/He statistic for marker filtering is described by Clark et al. (2022) <doi:10.1186/s12859-022-04635-9>. A variant calling pipeline for highly duplicated genomes is also included and is described by Clark et al. (2020, Version 1) <doi:10.1101/2020.01.11.902890>. Software Depends: R (>= 3.5.0), methods Imports: fastmatch, pcaMethods, Rcpp, stringi LinkingTo: Rcpp Suggests: rrBLUP, Rsamtools, GenomeInfoDb, Biostrings, GenomicRanges, VariantAnnotation, SummarizedExperiment, S4Vectors, IRanges, BiocGenerics, knitr, rmarkdown, GenomicFeatures, ggplot2, adegenet, txdbmaker, polymapR, BSgenome Lindsay V. Clark <Lindsay.Clark@seattlechildrens.org> Comprehensive R Archive Network (CRAN) Lindsay V. Clark [aut, cre] (ORCID: <https://orcid.org/0000-0002-3881-9252>), U.S. National Science Foundation [fnd] GPL (>= 2) 2026-03-22 application/tgz https://CRAN.R-project.org/package=polyRAD doi:10.32614/CRAN.package.polyRAD