Number and Brightness Image Analysis R package nandb version 2.1.1 Calculation of molecular number and brightness from fluorescence microscopy image series. The software was published in a 2016 paper <doi:10.1093/bioinformatics/btx434>. The seminal paper for the technique is Digman et al. 2008 <doi:10.1529/biophysj.107.114645>. A review of the technique was published in 2017 <doi:10.1016/j.ymeth.2017.12.001>. Software Depends: R (>= 3.1) Imports: assertthat, autothresholdr (>= 1.3.11), BBmisc, checkmate (>= 1.9.3), detrendr (>= 0.6.12), dplyr, filesstrings (>= 3.2), ggplot2, glue (>= 1.3), ijtiff (>= 2.2), magrittr (>= 1.5), purrr, Rcpp (>= 1.0.1), reshape2, rlang (>= 0.3.3), stringr (>= 1.4), utils, viridis, withr (>= 2.1.0) LinkingTo: Rcpp (>= 1.0.1) Suggests: abind, covr, gridExtra, knitr, magick, matrixStats (>= 0.50), pacman, rmarkdown, spelling, testthat (>= 2.1), tidyr Rory Nolan <rorynoolan@gmail.com> Comprehensive R Archive Network (CRAN) Rory Nolan [aut, cre, cph] (ORCID: <https://orcid.org/0000-0002-5239-4043>), Luis Alvarez [ctb, cph] (ORCID: <https://orcid.org/0000-0003-1316-1906>), Sergi Padilla-Parra [ctb, ths, cph] (ORCID: <https://orcid.org/0000-0002-8010-9481>) BSD_3_clause + file LICENSE (https://CRAN.R-project.org/package=nandb/LICENSE) 2025-05-06 application/tgz https://CRAN.R-project.org/package=nandb doi:10.32614/CRAN.package.nandb en-US