Four-Step Biodiversity Analysis Based on 'iNEXT' R package iNEXT.4steps version 1.0.1 Expands 'iNEXT' to include the estimation of sample completeness and evenness. The package provides simple functions to perform the following four-step biodiversity analysis: STEP 1: Assessment of sample completeness profiles. STEP 2a: Analysis of size-based rarefaction and extrapolation sampling curves to determine whether the asymptotic diversity can be accurately estimated. STEP 2b: Comparison of the observed and the estimated asymptotic diversity profiles. STEP 3: Analysis of non-asymptotic coverage-based rarefaction and extrapolation sampling curves. STEP 4: Assessment of evenness profiles. The analyses in STEPs 2a, 2b and STEP 3 are mainly based on the previous 'iNEXT' package. Refer to the 'iNEXT' package for details. This package is mainly focusing on the computation for STEPs 1 and 4. See Chao et al. (2020) <doi:10.1111/1440-1703.12102> for statistical background. Software Depends: R (>= 4.0) Imports: ggplot2, reshape2, dplyr, stats, ggpubr, purrr, iNEXT.3D Suggests: testthat, knitr, rmarkdown Anne Chao <chao@stat.nthu.edu.tw> Comprehensive R Archive Network (CRAN) Anne Chao [aut, cre], KaiHsiang Hu [ctb] GPL (>= 3) 2024-06-18 application/tgz https://CRAN.R-project.org/package=iNEXT.4steps doi:10.32614/CRAN.package.iNEXT.4steps