Guide Sequence Design for CRISPR/Cas9 R package crispRdesignR version 1.1.7 Designs guide sequences for CRISPR/Cas9 genome editing and provides information on sequence features pertinent to guide efficiency. Sequence features include annotated off-target predictions in a user-selected genome and a predicted efficiency score based on the model described in Doench et al. (2016) <doi:10.1038/nbt.3437>. Users are able to import additional genomes and genome annotation files to use when searching and annotating off-target hits. All guide sequences and off-target data can be generated through the 'R' console with sgRNA_Design() or through 'crispRdesignR's' user interface with crispRdesignRUI(). CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and the associated protein Cas9 refer to a technique used in genome editing. Software Depends: R (>= 2.10) Imports: Biostrings, gbm, GenomicRanges, BiocGenerics, IRanges, GenomeInfoDb, S4Vectors, rtracklayer, stringr, vtreat, shiny, DT Suggests: BSgenome.Scerevisiae.UCSC.sacCer3 Dylan Beeber <dylan.beeber@gmail.com> Comprehensive R Archive Network (CRAN) Dylan Beeber [aut, cre], Frederic Chain [aut] GPL-3 2023-04-12 application/tgz https://CRAN.R-project.org/package=crispRdesignR doi:10.32614/CRAN.package.crispRdesignR