| alphaBeta | Calculating a significance score of a gene based on the corresponding sgRNAs' p-values of the gene. |
| calculateGeneLFC | Calculating gene-level log fold ratios |
| calculateGenePval | Calculating gene level p-values using modified robust rank aggregation (alpha-RRA method) on sgRNAs' p-values |
| densityPlot | 2D density contour plot of gene log2 fold ratios against gene expression levels |
| EMFit | Fitting multi-component normal mixture models by R package mixtools |
| makeRhoNull | Generating the null distribution of the significance score of a gene. |
| mda231 | CRISPR screen data of cell line MDA-MB-231. |
| medianNormalization | Median normalization of sgRNA counts |
| normalMM | Performing empirical Bayes modeling on limma results |
| permuteLimma | Modeling CRISPR data with a permutation test between conditions by R package limma |
| preparePlotData | Prepare data for density plot and ridge plot |
| ridgePlot | Density ridgeline plot of gene expression levels for different FDR groups. |
| runLimma | Modeling CRISPR screen data by R package limma |
| scatterPlot | Scatter plot of log2 fold ratios against gene expression levels |